RNA Nanostructures by Eckart Bindewald & Bruce A. Shapiro

Author:Eckart Bindewald & Bruce A. Shapiro
Language: eng
Format: epub
Publisher: Springer New York, New York, NY

3.4 Gel Purification of RNA AsiC

1.Cast a polyacrylamide gel (1 × 180 × 250 mm, or similar) using 10% acrylamide (ratio of monomeric–dimeric acrylamide may range from 19:1 to 29:1) and 8 M urea in TBE. To start polymerization, add first 25 μL tetramethylethylenediamine (TEMED) then 150 μL 10% ammonium persulfate (APS) to 25 mL of the gel solution.

2.Set up the gel in the electrophoresis chamber, pour TBE buffer into the chamber and rinse the wells with a syringe.

3.Prerun gel at 20 W for at least 20 min (see Note 17 ).

Download

Copyright Disclaimer:
This site does not store any files on its server. We only index and link to content provided by other sites. Please contact the content providers to delete copyright contents if any and email us, we'll remove relevant links or contents immediately.